Before assessing the immunohistochemical staining results of an antibody in your experimental model, it's a good idea to perform optimization taking into account the antibody, model system, and protocol to determine the conditions for optimal antibody performance in your lab.
more helpful information to improve your IHC, download the download the Guide to Successful Immunohistochemistry.
You can also check out previous Learning Lab LIVE! sessions on IHC and other applications.
