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The Importance of Controls in Immunohistochemistry

 

The use of controls is crucial in any biological assay, but especially in immunohistochemistry, where there may be a number of variables and unknowns. Controls in IHC can help to determine if the staining observed is the result of the antibody binding specifically to its antigen or of off-target binding. Controls can also be used to determine if the protocol was performed successfully. This presentation includes:

  • Target controls: cells and tissue
  • Isotype controls: monoclonal and polyclonal
  • Secondary-only controls: endogenous biotin, pigment, and mouse-on-mouse controls
  • Peptide blocking controls for modification-specific antibodies
  • Phosphatase controls for phosphorylation-specific antibodies