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Validating Protein Interactions with Co-Immunoprecipitation Using Endogenous and Tagged Protein Models


Immunoprecipitation (IP) leverages the specificity of an antibody to enrich proteins in a sample and investigate its interactions with other macromolecules. This presentation includes a general workflow for co-immunoprecipitation (co-IP) and what you should consider before setting up your experiment, including:

  • Choice of model system;
  • Choice of cell lysis buffers and when to include a pre-clear step;
  • Primary antibody dilution and choice of beads;
  • How to mitigate IgG masking by heavy and light chains;
  • Using epitope tags for IP: advantages and key optimization considerations;
  • Controls for IP experiments targeting post-translational modifications.

For more expert guidance on setting up your IP experiment, watch this Tech Tips video: How to Select an Antibody for Immunoprecipitation