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Impacts of Lysis Buffer and Sonication on Western Blotting Outcomes

 

Early steps in the western blotting protocol involve the preparation of tissue or cell lysates prior to electrophoresis. Understanding the components of non-denaturing and denaturing lysis buffers , detergents, and protease/phosphatase inhibitors, as well as the benefits of a sonication step, can aid experimental design and the success of your western blot.