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Western Blot (WB) Experimental Design: Choosing Positive Controls


What's the difference between positive lysate controls and loading controls for Western Blot (WB)? Loading controls are used to verify proper sample handling and gauge the efficiency of electrophoretic transfer to the membrane. Positive lysate controls, on the other hand, give you a more biologically relevant idea of how much protein expression to expect in your experimental sample. Watch the video to learn more, including positive controls for total versus phospho specific antibodies.