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Get Technical Episode 1: Antibody Basics


The Get Technical series explains antibody technologies and the biology behind immunoassays. Learn how antibodies are made, how they work, and the pros and cons of various antibody types. Episode 1 compares monoclonal and polyclonal antibodies and introduces the 5 properties that affect their performance.

You may have noticed that a lot of people have been talking about antibody quality and antibody validation over the past few years. This is primarily in response to a number of questions that have been raised about the specificity and reproducibility of antibodies being used for research purposes. What are the advantages or disadvantages of using polyclonal or monoclonal antibodies in immunoassays? What are the pros and cons of using mouse or rabbit monoclonal antibodies? And what is a recombinant antibody?

We’ve assembled this video series to help clear the air about research antibodies, and help you understand how they are made, how they work (or don’t work), and the pros and cons of various antibody types. Today’s topic is “Antibodies Basics” in which we’ll discuss the fundamental biology of an antibody and the different types of antibodies commonly used in biomedical research.