IHC Tissue Controls & More

How do you know if your antibody is working properly in immunohistochemistry (IHC)? In this Tech Tip, Abbey discusses the use of positive and negative tissue controls, isotype controls, and controls for phospho-specific and modification-specific antibodies. No single assay is sufficient to demonstrate antibody specificity and sensitivity, but performing multiple experiments, including different controls, can give you more confidence in your IHC results.