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Western Blotting Protocol


Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. We understand that western blotting experiments are time consuming and critical to your research progress. For that reason, we provide optimized protocols along with reference information and technical support to make your western blotting experience successful.

Read the full Cell Signaling Technology Western Blot Protocol


  1. Intro
  2. Solutions and Reagents
  3. Sample Preparation and Protein Blotting
  4. Membrane Blocking and Antibody Incubation
  5. Protein Detection
  6. Importance of Following the Recommended Protocol
  7. Conclusion