Exceptional Performance of XP^® Monoclonal Antibodies

β-Catenin (D10A8) XP^® Rabbit mAb #8480: Western blot analysis (A) of extracts from various cell lines using #8480. Flow cytometric analysis (B) of NCI-H28 (blue) or HeLa (green) cells using #8480. Confocal IF analysis (C) of mouse colon using #8480 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye). IHC analysis (D) of frozen mouse colon using #8480. Chromatin immunoprecipitations (E) were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 20 μl of #8480 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human Axin2 Intron 1 Primers #8973, SimpleChIP^® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.