Product Pathways - Ca / cAMP / Lipid Signaling
SPHK1 (D1H1L) Rabbit mAb #12071
|12071S||100 µl (10 western blots)||---||In Stock||---|
|12071||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
SPHK1 (D1H1L) Rabbit mAb recognizes endogenous levels of total SPHK1 protein. This antibody also cross-reacts with a protein of unknown origin at 160 kDa in some cell lines.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro374 of human SPHK1 protein.
Western blot analysis of extracts from various cell lines using SPHK1 (D1H1L) Rabbit mAb.
Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.
- Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
- Xia, P. et al. (2000) Curr Biol 10, 1527-30.
- Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
- Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
- Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
- Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
- Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
- Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
- Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
- Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
- Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
- Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.
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