Product Pathways - Lymphocyte Signaling
CCR2 (D14H7) Rabbit mAb #12199
|12199S||100 µl (10 western blots)||---||In Stock||---|
|12199||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.
Western blot analysis of extracts from various cell lines using CCR2 (D14H7) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). HEL cells are reported to be negative for CCR2 expression (2).
CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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