Product Pathways - PI3K / Akt Signaling
YAP (1A12) Mouse mAb #12395
|12395S||100 µl (10 western blots)||---||In Stock||---|
|12395||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||65-75||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
YAP (1A12) Mouse mAb recognizes endogenous levels of total YAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to a fragment of human YAP1 protein.
Western blot analysis of extracts from various cell lines using YAP (1A12) Mouse mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded breast carcinoma using YAP (1A12) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using YAP (1A12) Mouse mAb.
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a critical and widely conserved role in regulating tissue growth and organ size. Upon phosphorylation at Ser127, YAP translocates to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (6-8).
- Sudol, M. (1994) Oncogene 9, 2145-2152.
- Mohler, P. et al. (1999) J. Cell Biol. 147, 879-890.
- Espanel, X. and Sudol, M. (2001) J. Biol. Chem. 276, 14514-14523.
- Sudol, M. et al. (1995) FEBS Lett. 369, 67-71.
- Yagi, R. et al. (1999) EMBO J. 18, 2551-2562.
- Basu, S. et al. (2003) Mol. Cell 11, 11-23.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
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