Product Pathways - CD Markers
CD82 (D7G6H) Rabbit mAb #12439
|12439S||100 µl (10 western blots)||---||In Stock||---|
|12439||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD82 (D7G6H) recognizes endogenous levels of total CD82 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 of human CD82 protein.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD82 (hCD82-Myc/DDK; +), using CD82 (D7G6H) Rabbit mAb.
Western blot analysis of extracts from various cell lines using CD82 (D7G6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded breast adenocarcinoma using CD82 (D7G6H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded cell pellets, HT-29 (left) and A549 (right), using CD82 (D7G6H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using CD82 (D7G6H)
Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using CD82 (D7G6H) Rabbit mAb.
CD82 (KAI1) belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). CD82 does not have enzymatic activity and appears to function by regulating the trafficking of other proteins and organization of the cell membrane (1). CD82 was originally described as a costimulator for T cells that directly associates with CD4 and CD8, and was subsequently identified during a screen as a metastasis suppressor in prostate cancer (2,3). CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies (4-6). CD82 suppresses metastasis through multiple mechanisms including inhibition of cell motility and invasion by modulating c-Met and the urokinase plasminogen activator surface receptor (uPAR), as well as promotion of homotypic cell-cell adhesion by stabilizing interactions between E-cadherin and β-catenin (7-9).
- Tsai, Y.C. and Weissman, A.M. (2011) FEBS Lett 585, 3166-73.
- Imai, T. et al. (1992) J Immunol 149, 2879-86.
- Dong, J.T. et al. (1995) Science 268, 884-6.
- Liu, F.S. et al. (2003) Clin Cancer Res 9, 1393-8.
- Yang, X. et al. (2001) Cancer Res 61, 5284-8.
- Lombardi, D.P. et al. (1999) Cancer Res 59, 5724-31.
- Sridhar, S.C. and Miranti, C.K. (2006) Oncogene 25, 2367-78.
- Bass, R. et al. (2005) J Biol Chem 280, 14811-8.
- Abe, M. et al. (2008) Cancer Lett 266, 163-70.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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