Product Pathways - Nuclear Receptor Signaling
Vitamin D3 Receptor (D2K6W) Rabbit mAb #12550
|12550S||100 µl (10 western blots)||---||In Stock||---|
|12550||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse||Endogenous||48, 54||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Species predicted to react based on 100% sequence homology: Hamster, Bovine, Pig, Horse.
Specificity / Sensitivity
Vitamin D3 Receptor (D2K6W) Rabbit mAb recognizes endogenous levels of total vitamin D3 receptor protein. This antibody does not cross-react with vitamin D3 receptor-like proteins. Based upon sequence alignment, this antibody is predicted to react with both VDRB1 and VDRB2 isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human vitamin D3 receptor isoform A protein.
Western blot analysis of extracts from various cell lines using Vitamin D3 Receptor (D2K6W) Rabbit mAb.
Western blot analysis of kidney tissue extracts from Vdr (+/+) and Vdr (-/-) mice using Vitamin D3 Receptor (D2K6W) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). (Vdr (+/+) and Vdr (-/-) kidneys were kindly provided by Dr. Marie Demay, Massachusetts General Hospital).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human vitamin D receptor isoform A (hVDRA-Myc/DDK; +), full-length human pregnane X receptor (hPXR-Myc/DDK; +), and full-length human constitutive androstane receptor (hCAR-Myc/DDK; +), using Vitamin D3 Receptor (D2K6W) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).
Immunoprecipitation of vitamin D3 receptor from T-47D cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Vitamin D3 Receptor (D2K6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Vitamin D3 Receptor (D2K6W) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vitamin D3 Receptor (D2K6W) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Vitamin D3 Receptor (D2K6W) Rabbit mAb.
Although originally identified based on their roles in calcium and bone homeostasis, the vitamin D3 receptor (VDR/NR1I1) and its ligand 1-α, 25-dihydroxycholecalciferol [1α, 25(OH)2D3] are now recognized to exert biological effects in almost every tissue of the human body. Targets for vitamin D signaling include the central nervous system, skin, immune system, endocrine glands, kidney, and colon. At the cellular level, vitamin D signaling affects proliferation, differentiation, and apoptosis of both normal and transformed cells. Within the steroid receptor gene family, VDR belongs to the NR1I subfamily that also includes NR1I2/PXR and NR1I3/CAR. The human VDR gene is composed of 11 exons that encode six domains (A-F) of the full length VDR protein, which includes an N-terminal dual zinc finger DNA binding domain, a C-terminal ligand-binding activity domain, and an extensive unstructured region that links the two functional domains together (1). Upon 1α, 25(OH)2D3 binding to the hormone ligand-binding domain, VDR is stabilized by the phosphorylation of Ser51 in the DNA-binding domain by PKC (2), and Ser208 in the hinge region by casein kinase II (3). VDR associates with the retinoic acid receptor (RXR) through dimerization domains. The 1α, 25(OH)2D3-VDR-RXR complex binds to the vitamin D response elements (VDREs) in the promoters of target genes through the DNA-binding domain. Ligand-induced conformation changes in VDR results in the dissociation of the co-repressor, silencing-mediator for retinoid and thyroid hormone receptors (SMRT), and allows interaction of the VDR activation function (AF2) transactivation domain with transcriptional coactivators (1).
Studies have shown that variable VDR expression is associated with different forms or stages of cancer and likely results from tissue-type variation in 1α, 25(OH)2D3 signaling. In the case of colon cancer, research indicates that VDR expression is relatively higher in hyperplastic colon polyps and during early tumorigenesis but diminishes in later stage, poorly differentiated tumors. Multiple studies suggest that 1α, 25(OH)2D3 may be an attractive target for development as a therapeutic anticancer agent (4,5) .
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3085 RXRα (D6H10) Rabbit mAb
- 6348 SignalSilence® RXRα siRNA I
- 8965 RARγ1 (D3A4) XP® Rabbit mAb
- 12719 SignalSilence® Vitamin D3 Receptor siRNA I
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9999 Nonfat Dry Milk
- 9863 Protein A Agarose Beads
- 3900 Rabbit (DA1E) mAb IgG XP® Isotype Control
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 8059 SignalStain® DAB Substrate Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.