Product Pathways - Ca / cAMP / Lipid Signaling
DAG Lipase β (D4P7C) Rabbit mAb #12574
|12574S||100 µl (10 western blots)||---||In Stock||---|
|12574||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
DAG Lipase β (D4P7C) Rabbit mAb recognizes endogenous levels of total DAG Lipase β protein. In some tissues, this antibody may detect a 48 kDa protein of unknown origin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human DAG Lipase β protein.
Western blot analysis of extracts from various cell lines using DAG Lipase β (D4P7C) Rabbit mAb Rabbit mAb.
Diacylglycerol (DAG) lipases comprise two enzymes called DAG lipase α and β, which are the products of two related genes (1). DAG lipases are transmembrane proteins composed of a short amino-terminal intracellular domain, four transmembrane domains, and a large carboxy-terminal cytoplasmic domain containing the active site. These enzymes are responsible for the biosynthesis of 2-acylglycerol from diacylglycerol in a calcium-dependent manner (1). One of the major endocannabinoid ligands that activate cannabinoid receptors, 2-arachidonyl glycerol (2-AG), is produced by DAG lipases (2). Research studies suggest that DAG lipase α is the isoform primarily responsible for the central production of 2-AG (3). DAG lipase β has been implicated in studies of 2-AG production at the periphery in specific cell types and pathophysiological contexts, such as in hepatic stellate cells during alcohol induced fatty liver (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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