Product Pathways - TGF-beta/Smad Signaling
Smad 1/5/8 Antibody Sampler Kit #12656
|12656S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb #5753||40 µl||W, IP||H||M, R, Mk||60||Rabbit IgG|
|Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516||40 µl||W, IF-IC, F||H, M, R||60||Rabbit IgG|
|Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody #9511||40 µl||W, IP, ChIP||H, M, R, Mi, X||60||Rabbit|
|Smad1 (D59D7) XP® Rabbit mAb #6944||40 µl||W, IP, IF-IC, F, ChIP||H, M, Mk||X, B||60||Rabbit IgG|
|Smad4 Antibody #9515||40 µl||W, ChIP||H, M, R, Mk||70||Rabbit|
|Smad5 (D4G2) Rabbit mAb #12534||40 µl||W, IP, ChIP||H, M, R, Mk||60||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mi=Mink, X=Xenopus, Mk=Monkey
Western blot analysis of extracts from various cell lines using Smad5 (D4G2) Rabbit mAb #12534.
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (60 mJ/cm2 for 2 min, 1.5 hr recovery; +), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb #5753 (upper) and Smad1 Antibody #9743 (lower).
Western blot analysis of extracts from various cell lines using Smad1 (D59D7) XP® Rabbit mAb #6944.
Western blot analysis of extracts from untransfected Xenopus cells and BMPRI/Smad1-transfected 293 cells, untreated (-) or BMP-4-treated (+), using Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody #9511. (Xenopus cells provided by Dr. Malcolm Whitman, Harvard University, Massachusetts.)
Western blot analysis of extracts from various cell lines using Smad4 Antibody #9515.
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated (-) or BMP-4-treated (+), using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516.
The Smad1/5/8 Antibody Sampler Kit provides an economical means of detecting target proteins of the BMP signaling pathway. The kit contains enough primary antibodies to perform four western blots with each.
Specificity / Sensitivity
Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total Smad1, Smad4, and Smad5 antibodies detect their respective targets at endogenous levels.
Source / Purification
Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser206 of human Smad1 protein or Ser463/465 of human Smad5. Total Smad1 and Smad5 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human Smad1 and Pro249 of human Smad5 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the residues surrounding Ser463/465 of human Smad5 or Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.
Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smads by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smads regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-7) .
- Horbelt, D. et al. (2012) Int J Biochem Cell Biol 44, 469-74.
- Ikushima, H. and Miyazono, K. (2010) Nat Rev Cancer 10, 415-24.
- Kitisin, K. et al. (2007) Sci STKE 2007, cm1.
- Schmierer, B. and Hill, C.S. (2007) Nat Rev Mol Cell Biol 8, 970-82.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Sapkota, G. et al. (2007) Mol Cell 25, 441-54.
- Alarcón, C. et al. (2009) Cell 139, 757-69.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.