Product Pathways - Cytoskeletal Signaling
Endosomal Marker Antibody Sampler Kit #12666
|12666S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Caveolin-1 (D46G3) XP® Rabbit mAb #3267||40 µl||W, IP, IHC-P, IF-IC, F||H, M, R, Hm, Mk, B, Dg||21, 24||Rabbit IgG|
|Clathrin Heavy Chain (D3C6) XP® Rabbit mAb #4796||40 µl||W, IP, IF-IC||H, M, R, Mk||Pg||190||Rabbit IgG|
|EEA1 (C45B10) Rabbit mAb #3288||40 µl||W, IP, IF-IC||H, M, R||170||Rabbit IgG|
|Rab5 (C8B1) Rabbit mAb #3547||40 µl||W, IF-IC||H, M, R, Mk||25||Rabbit IgG|
|Rab7 (D95F2) XP® Rabbit mAb #9367||40 µl||W, IP, IF-IC||H, M, R, Mk||23||Rabbit IgG|
|Rab11 (D4F5) XP® Rabbit mAb #5589||40 µl||W, IP, IF-IC||H, M, R, Mk||Hm, Pg||25||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey, B=Bovine, Dg=Dog
Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb #3267.
Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb #3288.
Western blot analysis of extracts from various cell lines using Rab5 (C8B1) Rabbit mAb #3547.
Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb #4796.
Western blot analysis of extracts from various cell lines using Rab11 (D4F5) XP® Rabbit mAb #5589.
Western blot analysis of extracts from various cell lines using Rab7 (D95F2) XP® Rabbit mAb #9367.
The Endosomal Marker Antibody Sampler Kit provides an economical means of distinguishing endosomes in the early, late, and recycling phases. The kit includes enough antibody to perform four western blot experiments with each primary antibody.
Specificity / Sensitivity
Each antibody will detect endogenous total levels of their target protein. The antibodies do not cross-react with other isoforms, with the exception of the Rab11 (D4F5) XP® Rabbit mAb which will detect both Rab11a and Rab11b.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1, Pro1663 of human clathrin heavy chain protein, Ser70 of human EEA1 protein, Gly190 of human Rab5A protein, Glu188 of human Rab7 protein, and near the amino terminus of human Rab11 protein.
Endosomes are formed by the invagination of the plasma membrane to form vesicles in an effort to recycle components of the cell (1). Endosomes can be coated in clathrin when vesicles form at clathrin-coated pits (2). Caveolins are 21-24 kDa integral proteins that interact with cholesterol and are the main structural components of the cholesterol/sphingolipid-enriched plasma membrane caveolae (3). Each stage of endosome maturation is marked by a unique set of proteins. EEA1 is an early endosome marker that is essential for membrane fusion and trafficking (4). Members of the ras superfamily of small Rab GTPases, specifically Rab5, Rab7, and Rab11 are markers of the early, late and recycling endosomes (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.