Product Pathways - Apoptosis
Caspase-7 (D2Q3L) Rabbit mAb #12827
|12827S||100 µl (10 western blots)||---||In Stock||---|
|12827||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||20, 35||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Caspase-7 (D2Q3L) Rabbit mAb recognizes endogenous levels of total caspase-7 protein. This antibody detects both the full-length (35 kDa) and large (20 kDa) subunits following caspase-7 activation.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro158 of human caspase-7 protein.
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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