Product Pathways - TGF-beta/Smad Signaling
SIX1 (D4A8K) Rabbit mAb #12891
|12891S||100 µl (10 western blots)||---||In Stock||---|
|12891||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||36||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
SIX1 (D4A8K) Rabbit mAb recognizes endogenous levels of total SIX1 protein. It does not cross-react with other SIX family proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro249 of human SIX1 protein.
Western blot analysis of extracts from various cell lines using SIX1 (D4A8K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of A-204 (positive, left) and Caco-2 (negative, right) cells, using SIX1 (D4A8K) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).
Immunoprecipitation of SIX1 protein from A-204 cell extracts using Rabbit DA1E mAb IgG XP® Isotype Control #3900 (lane 2) or SIX1 (D4A8K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SIX1 (D4A8K) Rabbit mAb.
Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in Drosophila mutant screens for embryonic eye development genes (1-3). The prototypical family member (sine oculis, so) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans (SIX1) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7).
In contrast to the Drosophila ortholog, the vertebrate SIX1 gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).
- Kumar, J.P. (2009) Cell Mol Life Sci 66, 565-83.
- Fischbach, K.F. and Technau, G. (1984) Dev Biol 104, 219-39.
- Fischbach, K.F. and Heisenberg, M. (1981) Proc Natl Acad Sci U S A 78, 1105-9.
- Boucher, C.A. et al. (1996) Genomics 33, 140-2.
- Pignoni, F. et al. (1997) Cell 91, 881-91.
- Ford, H.L. et al. (1998) Proc Natl Acad Sci U S A 95, 12608-13.
- Coletta, R.D. et al. (2004) Proc Natl Acad Sci U S A 101, 6478-83.
- Oliver, G. et al. (1995) Development 121, 4045-55.
- Kaiser, R. et al. (2007) Am J Med Genet A 143A, 2185-8.
- Fujimoto, Y. et al. (2013) Dev Cell 26, 416-30.
- Liu, Y. et al. (2013) PLoS One 8, e67762.
- Behbakht, K. et al. (2007) Cancer Res 67, 3036-42.
- Ng, K.T. et al. (2006) Br J Cancer 95, 1050-5.
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