Product Pathways - Growth Factors/Cytokines
IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) #12912
|12912S||100 µl (10 western blots)||---||In Stock||---|
|12912P||40 µl (4 western blots)||---||In Stock||---|
|12912||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total IL-6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant mouse IL-6 protein.
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation; +), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of recombinant Mouse His6Interleukin-6 (mHis6IL-6) #8857 using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).
Immunoprecipitation of IL-6 from Raw 264.7 cell extracts treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) (green) using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific). All cells were incubated with Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells (1,2). IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated (3). The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα (3). Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
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Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.