Product Pathways - Metabolism
Catalase (D4P7B) XP® Rabbit mAb #12980
|12980S||100 µl (10 western blots)||---||In Stock||---|
|12980P||40 µl (4 western blots)||---||In Stock||---|
|12980||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Catalase (D4P7B) XP® Rabbit mAb recognizes endogenous levels of total catalase protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human catalase protein.
Western blot analysis of extracts from Hep G2, HeLa, and 293 cells using Catalase (D4P7B) XP® Rabbit mAb.
Immunoprecipitation of catalase from extracts of HeLa cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Catalase (D4P7B) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Catalase (D4P7B) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using Catalase (D4P7B) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen (1). Research studies show that overexpression of this antioxidant enzyme increases the ability of pancreatic β-cells to scavenge reactive oxygen species (ROS), thereby protecting pancreatic β-cells from oxidative stress (2). The pancreatic β-cells overexpressing this enzyme are also protected from hydrogen peroxide-mediated lipotoxicity, providing further evidence for the importance of catalase in the pathogenesis of diabetes (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.