Product Pathways - NF-kB Signaling
SARM1 (D2M5I) Rabbit mAb #13022
|13022S||100 µl (10 western blots)||---||In Stock||---|
|13022||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SARM1 (D2M5I) Rabbit mAb recognizes endogenous levels of total human SARM1 protein and transfected levels of total mouse SARM1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro324 of human SARM1 protein.
Western blot analysis of extracts from various cell lines using SARM1 (D2M5I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse Sarm1b (mSarm1b; +), using SARM1 (D2M5I) Rabbit mAb.
Confocal immunofluorescent analysis of ACHN (left) and HaCaT (right) cells using SARM1 (D2M5I) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
Sterile alpha and TIR motif-containing protein 1 (SARM1) is a TIR domain-containing adaptor protein that contains two sterile alpha motif (SAM) domains (15). SARM1 is the only known TIR domain-containing adaptor that does not activate NF-κB, but instead negatively regulates toll-like receptor signaling (16). Research studies suggest that SARM1 inhibits signaling by TLR3 and TLR4 through direct interaction with the TIR domain-containing adapter TRIF, which is required for TLR3 and MyD88-independent TLR4 signaling (16-18). Additional research indicates that SARM1 can mediate injury-induced axon death, neuronal cell death in response to infection with the encephalitis-causing La Crosse virus, and T cell death following an immune response to infection (19-21).
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