Product Pathways - Metabolism
LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) #13031
|13031S||100 µl (250 slides)||---||In Stock||---|
|13031P||40 µl (100 slides)||---||In Stock||---|
|13031||carrier free and custom formulation / quantity||email request|
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|IHC-P||1:250||Human, Mouse, Monkey||Endogenous||54||Rabbit IgG|
Species cross-reactivity is determined by the application specified.
Applications Key: IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total LKB1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LKB1 protein.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded cell pellets, LKB1 positive COS-7 (left) and LKB1 negative A549 (right), using LKB1 (D60C5F10) Rabbit mAb (IHC-Formulated).
LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).
Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).
- Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
- Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
- Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
- Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
- Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
- Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
- Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.
- Nakada, Y. et al. (2013) PLoS One 8, e73449. Applications: IHC-P (paraffin).
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For Research Use Only. Not For Use In Diagnostic Procedures.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.