Product Pathways - Cytoskeletal Signaling
Keratin 20 (D9Z1Z) XP® Rabbit mAb #13063
|13063S||100 µl (10 western blots)||---||In Stock||---|
|13063P||40 µl (4 western blots)||---||In Stock||---|
|13063||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
Keratin 20 (D9Z1Z) XP® Rabbit mAb recognizes endogenous levels of total keratin 20 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 20 protein.
Western blot analysis of extracts from various cell lines using Keratin 20 (D9Z1Z) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8547 (lower).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Keratin 20 (D9Z1Z) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded HT-29 (left) or MCF7 (right) cell pellets using Keratin 20 (D9Z1Z) XP® Rabbit mAb.
Flow cytometric analysis of MCF7 (blue) and HT-29 (green) cells using Keratin 20 (D9Z1Z) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Keratin 20 is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel cells (7). Research studies have shown that keratin 20 is an important marker of colon, liver, pancreatic, Merkel cell, and gastric cancer (8).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
- Moll, R. et al. (1992) Am J Pathol 140, 427-47.
- Karantza, V. (2011) Oncogene 30, 127-38.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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