Product Pathways - Metabolism
CTR1/SLC31A1 Antibody #13086
|13086S||100 µl (10 western blots)||---||In Stock||---|
|13086||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||26-34||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
CTR1/SLC31A1 Antibody recognizes endogenous levels of total CTR1 (SLC31A1) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human CTR1 (SLC31A1) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Caco-2 and SK-MEL-3 cells using CTR1/SLC31A1 Antibody.
The high affinity copper uptake protein 1 (CTR1, SLC31A1) helps maintain copper homeostasis by mediating dietary copper intake chiefly in the small intestine (1). A series of methionine-rich repeats and other residues are conserved among CTR1 genes across taxa, and are thought to be important for copper transport (2,3). In mammalian cells, CTR1 is localized to the plasma membrane and intracellular vesicles (3). Upon copper uptake via plasma membrane into cells, CTR1 is down regulated by clathrin-dependent endocytosis and degradation of CTR1 protein (4). Research studies suggest that the CTR1 copper transporter also mediates uptake of the anticancer drug cisplatin in yeast and mammals and that decreased CTR1 can result in the development of cisplatin resistance (5,6). Treatment of cancer cells with cisplatin can result in reduced CTR1 expression, which reduces cisplatin accumulation within cells and leads to cisplatin resistance in some human cancer cells (7-9).
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- Kim, B.E. et al. (2008) Nat Chem Biol 4, 176-85.
- Lee, J. et al. (2002) J Biol Chem 277, 4380-7.
- Petris, M.J. et al. (2003) J Biol Chem 278, 9639-46.
- Ishida, S. et al. (2002) Proc Natl Acad Sci U S A 99, 14298-302.
- Kuo, M.T. et al. (2007) Cancer Metastasis Rev 26, 71-83.
- Abada, P. and Howell, S.B. (2010) Met Based Drugs 2010, 317581.
- Yu, L. et al. (2011) Nan Fang Yi Ke Da Xue Xue Bao 31, 801-4.
- Kalayda, G.V. et al. (2012) J Inorg Biochem 116, 1-10.
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