Product Pathways - Protein Translation
Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb #13175
|13175S||100 µl (10 western blots)||---||In Stock||---|
|13175P||40 µl (4 western blots)||---||In Stock||---|
|13175||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||40||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb recognizes endogenous levels of PRAS40 protein only when phosphorylated at Thr246.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40 protein.
Western blot analysis of extracts from serum starved HeLa cells, untreated (-), insulin-treated (1 μM, 30 min; +), or insulin and λ phosphatase-treated (+), using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right) using Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb. Cells used are from the SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101.
Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
SignalSlide® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.