Product Pathways - Neuroscience
Calbindin (D1I4Q) XP® Rabbit mAb #13176
|13176S||100 µl (10 western blots)||---||In Stock||---|
|13176P||40 µl (4 western blots)||---||In Stock||---|
|13176||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||28||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Calbindin (D1I4Q) XP® Rabbit mAb recognizes endogenous levels of total calbindin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human calbindin protein.
Western blot analysis of extracts from various cell lines using Calbindin (D1I4Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of calbindin from mouse brain extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or Calbindin (D1I4Q) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Calbindin (D1I4Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymph node using Calbindin (D1I4Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse kidney using Calbindin (D1I4Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat brain using Calbindin (D1I4Q) XP(R) Rabbit mAb.
Confocal immunofluorescent analysis of normal rat brain using Calbindin (D1I4Q) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.