Product Pathways - Adhesion
Phospho-α-E-Catenin (Ser655/Thr658) Antibody #13231
|13231S||100 µl (10 western blots)||---||In Stock||---|
|13231||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||100||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-α-E-Catenin (Ser655/Thr658) Antibody recognizes endogenous levels of α-E-catenin protein only when phosphorylated at Ser655 and Thr658.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser655/Thr658 of human α-E-catenin protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell lines using Phospho-α-E-Catenin (Ser655/Thr658) Antibody.
Western blot analysis of extracts from NCI-H3255 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-α-E-Catenin (Ser655/Thr658) Antibody (upper), α-E-Catenin (23B2) Rabbit mAb #3240 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of phospho-α-E-catenin protein from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-α-E-Catenin (Ser655/Thr658) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-α-E-Catenin (Ser655/Thr658) Antibody.
Phospho-α-E-Catenin (Ser655/Thr658) Antibody specificity was determined by peptide competition ELISA. The binding of Phospho-α-E-Catenin (Ser655/Thr658) Antibody to phospho-α-E-catenin (Ser655/Thr658) peptide in the presence of increasing concentrations of competitor peptides is shown. Only the di-phospho-α-E-catenin (Ser655/Thr658) peptide competes for binding.
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).
Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
Phosphorylation of α-E-catenin at Ser655 and Thr658 are post-translational modifications identified in a number of mass spectrometry studies, using a variety of tissue and cell types of both mouse and human origin (5-8).
- Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
- Yamada, S. et al. (2005) Cell 123, 889-901.
- Drees, F. et al. (2005) Cell 123, 903-15.
- Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.
- Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
- Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13.
- Huttlin, E.L. et al. (2010) Cell 143, 1174-89.
- Pan, C. et al. (2008) Proteomics 8, 4534-46.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.