Product Pathways - NF-kB Signaling
Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb #13346
|13346S||100 µl (10 western blots)||---||In Stock||---|
|13346||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||65||Mouse IgG2b|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb recognizes endogenous levels of NF-κB p65 protein only when phosphorylated at Ser536.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB protein.
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (1 μg/ml, 15 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower).
Western blot analysis of extracts from HeLa and PC-3 cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (hTNF-α; 20 ng/ml, 5 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower).
Immunoprecipitation of phospho-NF-κB p65 (Ser536) from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 5 min) using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033.
Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
- Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
- Haskill, S. et al. (1991) Cell 65, 1281-9.
- Thompson, J.E. et al. (1995) Cell 80, 573-82.
- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
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