Product Pathways - DNA Damage
Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb #13439
|13439S||100 µl (10 western blots)||---||In Stock||---|
|13439||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein.
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (100 mJ, 1 hr recovery; +), using Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (upper) or PCNA (PC10) Mouse mAb #2586 (lower).
Immunoprecipitation of Ubiquityl-PCNA (Lys164) from UV-treated (100 mJ, 1 hr recovery) HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb.
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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