Product Pathways - Metabolism
LXR-β (D6M9D) Rabbit mAb #13519
|13519S||100 µl (10 western blots)||---||In Stock||---|
|13519||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat||Endogenous||63||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
LXR-β (D6M9D) Rabbit mAb recognizes endogenous levels of total LXR-β protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LXR-β protein.
Western blot analysis of extracts from various cell lines using LXR-β (D6M9D) Rabbit mAb.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human LXR-α protein (hLXR-α-Myc/DDK; +) or Myc/DDK-tagged full-length human LXR-β protein (hLXR-β-Myc/DDK; +), using LXR-β (D6M9D) Rabbit mAb (upper), DYKDDDDK (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and up-regulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson disease model (6).
- Repa, J.J. et al. (2000) Genes Dev 14, 2819-30.
- Willy, P.J. et al. (1995) Genes Dev 9, 1033-45.
- Teboul, M. et al. (1995) Proc Natl Acad Sci U S A 92, 2096-100.
- Mitro, N. et al. (2007) Nature 445, 219-23.
- Gabbi, C. et al. (2010) Proc Natl Acad Sci U S A 107, 14763-8.
- Dai, Y.B. et al. (2012) Proc Natl Acad Sci U S A 109, 13112-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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