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U2AF1 (D6S3Q) Rabbit Monoclonal Antibody #13705

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    U2AF1 (D6S3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total U2AF1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Hamster, Zebrafish, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala47 of human U2AF1 protein.

    Background

    U2 small nuclear RNA auxiliary factor 1 (U2AF1) is the small (35 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF1 forms a heterodimer with the large (65 kDa) U2AF2 subunit to create the U2 auxiliary factor that recognizes the 3' splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3'-splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polyprimidine tract upstream of the 3’ splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in the corresponding U2AF1 gene are associated with a type of hematopoietic stem cell disorder known as myelodysplastic syndrome (MDS), which can be characterized by low blood counts, anemia, and enhanced acute myeloid leukemia risk (7-9). Somatic U2AF1 mutations frequently affect highly conserved zinc finger protein regions that result in defective pre-mRNA splicing of genes involved in cell cycle progression and RNA processing pathways, contributing to MDS pathogenesis (7,10).

    Alternate Names

    DKFZp313J1712; FP793; RN; RNU2AF1; Splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35 kDa subunit-like protein; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxiliary factor 1 like 5; U2 small nuclear RNA auxiliary factor 1-like protein 5; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF1; U2AF1L5; U2AF35; U2AFBP

    For Research Use Only. Not for Use in Diagnostic Procedures.
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