Render Target: STATIC
Render Timestamp: 2024-10-31T10:03:49.669Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-20 06:21:56.038
Product last modified at: 2024-05-30T07:16:14.702Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

USP39 Antibody #19758

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 65
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    USP39 Antibody recognizes endogenous levels of total USP39 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val88 of human USP39 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Ubiquitin specific protease 39 (USP39) is a 65 kDa protein that plays an important role in pre-mRNA splicing, as well as mitotic spindle formation. It displays significant homology with ubiquitin C-terminal hydrolase proteins (UCHs), containing both an N-terminal zinc finger domain as well as UCH-1 and UCH-2-like domains also observed in the UCH2 family of proteins (1). However, USP39 lacks a catalytic cysteine residue found in UCHs and has been shown experimentally to lack peptidase activity (2). USP39 associates with the U4/U6-U5 tri-small nuclear ribonucleoprotein (U4/U6-U5 tri-snRNP) complex and is necessary for the formation of the mature spliceosome. Silencing of USP39 has been shown to adversely affect chromosome segregation and cytokinesis in U2OS cells, likely due to improper splicing of Aurora B and other mRNAs necessary for mitotic spindle formation and checkpoint function (2). In addition, USP39 has been found to be overexpressed in many types of cancers, and in most cases is associated with tumor progression and poor prognosis. Overexpression has been observed in pancreatic (3), prostate (4), colorectal (5,6), lung (6,7), gastric (8), and triple negative breast cancers (9), as well as melanoma (10) and hepatocellular carcinoma (11,12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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