Product Pathways - Metabolism
ATGL (30A4) Rabbit mAb #2439
|2439S||100 µl (10 western blots)||---||In Stock||---|
|2439||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ATGL (30A4) Rabbit mAb detects endogenous levels of total ATGL protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL.
Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining of adipocytes, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brown fat, using ATGL (30A4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung, showing staining specific to adipocytes, using ATGL (30A4) Rabbit mAb.
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).
- Holm, C. et al. (1988) Science 241, 1503-1506.
- Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
- Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
- Zimmermann, R. et al. (2004) Science 306, 1383-1386.
- Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
- Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.
- Wang, H. et al. (2011) J Biol Chem 286, 15707-15. Applications: Western Blotting, IP.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
BODIPY® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.