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  3. SARS-CoV-1 Spike Protein (E7C5Y) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SARS-CoV-1 Spike Protein (E7C5Y) Rabbit Monoclonal Antibody #26769

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  • WB

    Product Specifications

    REACTIVITY Vir
    SENSITIVITY Transfected Only
    MW (kDa) 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-1 Spike Protein (E7C5Y) Rabbit Monoclonal Antibody recognizes transfected levels of total SARS-CoV-1 spike protein. This antibody does not cross-react with spike proteins from SARS-CoV-2 or MERS coronaviruses.

    Species Reactivity:

    Virus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of SARS-CoV-1 spike protein.

    Background

    The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of viruses (2). The genome of SARS-CoV-1 is similar to other coronaviruses, and is comprised of four key structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (3). Coronavirus spike proteins are class I fusion proteins that harbor an ectodomain, a transmembrane domain, and an intracellular tail (4,5). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at the S1/S2 junction, with possible secondary cleavage occurring at a downstream site (S2/S2’) (6). S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (3,5). As with SARS-CoV-2, the SARS-CoV-1 spike protein utilizes the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (7-9). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (10,11).

    Alternate Names

    E2; E2 glycoprotein precursor; Peplomer protein; S glycoprotein; SARS-CoV spike protein; sars2; spike glycoprotein; SPIKE_CVHSA

    Database Links

    UniProt ID: P59594


    Entrez-Gene Id: 1489668


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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