Product Pathways - NF-kB Signaling
IKKγ Antibody #2685
|2685S||100 µl (10 western blots)||---||In Stock||---|
|2685P||40 µl (4 western blots)||---||In Stock||---|
|2685||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||48||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
IKKγ Antibody detects endogenous levels of total IKKγ protein independent of phosphorylation. It does not cross-react with IKKα or IKKβ.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human IKKγ protein. Antibodies are purified by protein A and peptide affinity chromatography.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
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- Sarkar, S. et al. (2011) Mol Cell Biol 31, 602-15. Applications: Western Blotting.
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