Product Pathways - Cell Cycle / Checkpoint
ATRIP Antibody #2737
|2737S||100 µl (10 western blots)||---||In Stock||---|
|2737||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||82 (isoform 1)||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ATRIP Antibody detects endogenous levels of total ATRIP protein and targets the coiledcoil domain. All three major isoforms produced by alternative splicing are recognized
(isoforms 1, 2, and 3).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly267 of human ATRIP. Antibodies are purified by protein A and peptide affinity chromatography.
In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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