Product Pathways - Tyrosine Kinase / Adaptors
Phospho-c-Abl (Tyr245) Antibody #2861
|2861S||100 µl (10 western blots)||---||In Stock||---|
|2861||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||135 (c-Abl); 210 (Bcr-Abl)||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse.
Specificity / Sensitivity
Phospho-c-Abl (Tyr245) Antibody detects endogenous levels of c-Abl only when phosphorylated at tyrosine 245. The antibody cross-reacts with activated EGF receptors and PDGF receptors.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr245 of human c-Abl.
The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
- Ganapathipillai, S.S. et al. (2008) Cancer Res 68, 5769-77. Applications: Western Blotting.
- Goss, V.L. et al. (2006) Blood 107, 4888-97. Applications: Western Blotting.
- Smith, K. M. et al. (2003) Mol. Cell 12, 27-37. Applications: Western Blotting.
- Komatsu, N. et al. (2002) J.Biol.Chem. 278 (8), 6411-6419. Applications: Western Blotting.
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