Product Pathways - Cytoskeletal Signaling
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141
|3141L||300 µl (30 western blots)||---||In Stock||---|
|3141S||100 µl (10 western blots)||---||In Stock||---|
|3141||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||75 Moesin. 80 Ezrin, Radixin.||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Xenopus.
Specificity / Sensitivity
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at threonine 567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin. Antibodies are purified by protein A and peptide affinity chromatography.
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
- Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
- Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
- Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
- Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
- Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
- Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.
- Wu, K. L. et al. (2004) . J. Biol. Chem. 279 (25), 26280-26286. Applications: Western Blotting.
- John, G. R. et al. (2004) . J. Neurosci. 24 (11), 2837-2845. Applications: Western Blotting.
- Fievet, B. T. et al. (2004) . J. Cell Biol. 164 (5), 653-659. Applications: Western Blotting.
- Ren, L. et al. (2009) Oncogene 28, 792-802. Applications: Western Blotting.
- Li, Y. et al. (2007) J Immunol 178, 1938-47. Applications: Western Blotting.
- Chiba, H. et al. (2006) J Cell Biol 175, 971-80. Applications: Western Blotting.
- Coffey, G.P. et al. (2009) J Cell Sci 122, 3137-44. Applications: Western Blotting.
- Duterme, C. et al. (2009) J Biol Chem 284, 33495-508. Applications: Western Blotting.
- Canals, D. et al. (2010) J Biol Chem 285, 32476-85. Applications: Western Blotting.
- Baeyens, N. et al. (2010) Am J Physiol Cell Physiol 299, C1530-40. Applications: Western Blotting.
- Nakaya, M. et al. (2013) Nat Commun 4, 1532. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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