Phospho-Pyk2 (Tyr402) Antibody #3291
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 116 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L.T. et al. (2001) J Biol Chem 276, 7484-92.
- Watson, J.M. et al. (2001) J Biol Chem 276, 3536-42.
- Tang, H. et al. (2002) J Biol Chem 277, 5441-7.
- Berg, N.N. and Ostergaard, H.L. (1997) J Immunol 159, 1753-7.
- Ganju, R.K. et al. (1997) J Exp Med 185, 1055-63.
- Katagiri, T. et al. (2000) J Biol Chem 275, 19645-52.
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