Product Pathways - Lymphocyte Signaling
CD19 Antibody #3574
|3574S||100 µl (10 western blots)||---||In Stock||---|
|3574P||40 µl (4 western blots)||---||In Stock||---|
|3574||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
CD19 Antibody detects endogenous levels of CD19 independent of its phosphorylated state.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around the carboxy-terminus of human CD19. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of SDS extracts from untreated or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells and untreated or anti-mouse IgG treated (30 µg/ml for 2 minutes) A20 cells, using control CD19 Antibody.
Flow cytometric analysis of Jurkat T cells (blue) and Ramos B cells (green), using CD19 Antibody.
Confocal immunofluorescent analysis of Ramos (+) (left) and Jurkat (-) (right) cells using CD19 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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