Product Pathways - Cytoskeletal Signaling
ROCK1 (C8F7) Rabbit mAb #4035
|4035S||100 µl (10 western blots)||---||In Stock||---|
|4035||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||160||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
ROCK1 (C8F7) Rabbit mAb detects endogenous levels of total ROCK1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human ROCK1.
Western blot analysis of extracts from various cell types using ROCK1 (C8F7) Rabbit mAb.
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).
- Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
- Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
- Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
- Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
- Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
- Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
- Mayanagi, T. et al. (2008) J Biol Chem 283, 31183-96. Applications: Western Blotting.
- Park, J.H. et al. (2011) J Biol Chem 286, 23132-41. Applications: Western Blotting.
- Hudson, C.A. et al. (2012) Mol Hum Reprod 18, 265-79. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.