Name: HMGCS2 (D8B5M) Rabbit mAb
Price: 327.00 USD
Availability: InStock

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WB

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	50
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HMGCS2 (D8B5M) Rabbit mAb recognizes endogenous levels of total HMGCS2 protein. This antibody does not cross-react with HMGCS1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human HMGCS2 protein.

Background

Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) generates hydroxymethylglutaryl-CoA (HMG-CoA) from acetyl-CoA and acetoacetyl-CoA, a rate-limiting step in ketogenesis (1). Starvation or a high-fat and low-carbohydrate diet increases the levels of hepatic FGF21, which in turn up-regulates HMGCS2 expression (2). Furthermore, mTORC1 inhibition was shown to be required for the increase of HMGCS2 expression mediated by PPARα in response to fasting (3). In addition, studies on mice lacking HMGCS2 suggest that ketogenesis plays a role in the prevention of diet-induced fatty liver injury and hyperglycemia (4).

Database Links

UniProt ID: P54868

Entrez-Gene Id: 3158

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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HMGCS2 (D8B5M) Rabbit mAb #40364