Render Target: STATIC
Render Timestamp: 2024-10-31T09:52:13.444Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:57:56.307
Product last modified at: 2024-09-30T08:01:42.018Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Glut3 (E7M7V) Rabbit mAb #40538

Filter:
  • WB
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:3200 - 1:12800
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Glut3 (E7M7V) Rabbit mAb recognizes endogenous levels of total Glut3 protein. This antibody does not cross-react with Glut1, Glut2, or Glut4.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala472 of human Glut3 protein.

    Background

    Glucose transporter 3 (Glut3, also known as SLC2A3) has a higher affinity for glucose than the ubiquitous glucose transporter Glut1. In the normal brain, Glut3 expression is limited to neurons, whereas Glut1 is expressed in both neurons and glia. Glioblastoma-derived brain tumor initiating cells (BTICs) express increased levels of Glut3, enabling their survival in the microenvironment deficient in glucose. In addition, Glut3 expression levels correlate with poor survival in brain tumors (1). Furthermore, integrin αvβ3 was shown to stimulate PAK4-YAP/TAZ signaling to upregulate Glut3 expression in glioblastoma cells. A subpopulation of glioblastoma was identified to be addicted to Glut3 and is highly sensitive to the disruption of integrin αvβ3-PAK4-YAP/TAZ signaling (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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