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E-Tag (D1U2Q) Rabbit Monoclonal Antibody #41729

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    E-Tag (D1U2Q) Rabbit Monoclonal Antibody recognizes transfected levels of recombinant proteins containing an E-tag.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic E-tag peptide.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    E-tag is an epitope tag composed of a 13 residue peptide. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1,2).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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