Product Pathways - Lymphocyte Signaling
AML1 Antibody #4334
|4334S||100 µl (10 western blots)||---||In Stock||---|
|4334||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
AML1 antibody detects endogenous levels of total AML1 protein and the AML1/ETO fusion protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human AML1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Jurkat and Kasumi-1 cells, using AML1 Antibody.
Flow cytometric analysis of Jurkat cells, using AML1 Antibody (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent image of Jurkat cells labeled with AML1 Antibody (green). Mitochondria have been labeled with MitoTracker Red CMXRos.
AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
- Wang, S. et al. (1993) Mol Cell Biol 13, 3324-3339.
- Ogawa, E. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 6859-6863.
- Okuda, T. et al. (1996) Cell 84, 321-30.
- Wang, Q. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3444-3449.
- North, T.E. et al. (2004) Stem Cells 22, 158-168.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-387.
- Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
- Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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