nSMase2 (F4E1D) Rabbit Monoclonal Antibody #44083
- WB
- IHC
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
nSMase1, also known as SMPD2, is a Mg++-dependent neutral SMase widely expressed and predominantly localized to the endoplasmic reticulum (3,4). This protein has also been shown to have lyso-platelet-activating factor (PAF) phospholipase C activity (5). A second neutral SMase, nSMase2, also known as SMPD3, is predominantly expressed in the brain (6). The activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuli (1). nSMase2 has been suggested to regulate extracellular vesicle (EV) biogenesis, release, and uptake in the brain following injury (7,8). Analysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity, with developmental defects observed with loss of nSMase2 (9,10).
- Marchesini, N. and Hannun, Y.A. (2004) Biochem Cell Biol 82, 27-44.
- Ruvolo, P.P. (2001) Leukemia 15, 1153-60.
- Tomiuk, S. et al. (1998) Proc Natl Acad Sci USA 95, 3638-43.
- Tomiuk, S. et al. (2000) J Biol Chem 275, 5710-7.
- Sawai, H. et al. (1999) J Biol Chem 274, 38131-9.
- Hofmann, K. et al. (2000) Proc Natl Acad Sci USA 97, 5895-900.
- Lecuyer, M. et al. (2021) Neurobiol Dis 157, 105431.
- Tallon, C. et al. (2021) Biochem Pharmacol 194, 114796.
- Zumbansen, M. and Stoffel, W. (2002) Mol Cell Biol 22, 3633-8.
- Stoffel, W. et al. (2005) Proc Natl Acad Sci USA 102, 4554-9.
Alternate Names
FLJ22593; MGC138443; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; NSMA2; nSMase-2; nSMase2; SMPD3; Sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
Limited Uses
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