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Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805

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  • WB

Inquiry Info. # 4805

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    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 145
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb detects endogenous levels of SMC1 only when phosphorylated at serine 957. This antibody may also recognize phosphorylated human DNA-PKcs (450kDa).

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1.

    Background

    Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

    Alternate Names

    CDLS2; DKFZp686L19178; DXS423E; EIEE85; epididymis secretory sperm binding protein; KIAA0178; MGC138332; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1A; SMC1alpha; SMC1L1; SMCB; structural maintenance of chromosomes 1A; Structural maintenance of chromosomes protein 1A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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