Product Pathways - Ca / cAMP / Lipid Signaling
RACK1 (D59D5) Rabbit mAb #5432
|5432S||100 µl (10 western blots)||---||In Stock||---|
|5432||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster||Endogenous||32||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
RACK1 (D59D5) Rabbit mAb recognizes endogenous levels of total RACK1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein.
Western blot analysis of extracts from various cell types using RACK1 (D59D5) Rabbit mAb.
The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).
- Ron, D. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 839-843.
- Lopez-Bergami , P. et al. (2005) Mol. Cell 19, 309-320.
- McCahill, A. et al. (2002) Mol. Pharmacol. 62, 1261-1273.
- Nilsson, J. et al. (2004) EMBO Rep. 5, 1137-1141.
- Chang, B. Y. et al. (2002) Oncogene 21, 7619-7629.
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