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NBR1 (D2E6) Rabbit mAb (PE Conjugate) #54516

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Inquiry Info. # 54516

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated NBR1 (D2E6) Rabbit mAb #9891.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    NBR1 (D2E6) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total NBR1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys601 of human NBR1 protein.

    Background

    Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in selective autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5-11).

    Alternate Names

    1A1-3B; 1A13B; B-box protein; Cell migration-inducing gene 19 protein; FLJ55359; FLJ98272; IAI3B; KIAA0049; M17S2; Membrane component chromosome 17 surface marker 2; membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); MIG19; migration-inducing protein 19; NBR1; NBR1 autophagy cargo receptor; neighbor of BRCA1 gene 1; Neighbor of BRCA1 gene 1 protein; Next to BRCA1 gene 1 protein; Protein 1A1-3B

    For Research Use Only. Not for Use in Diagnostic Procedures.
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